Ultrastructural and nanomechanical changes of the cornea following enzymatic degradation
Vol. 2 No. 2 (2018), ESB ITA
Vol. 2 No. 2 (2018)

Ultrastructural and nanomechanical changes of the cornea following enzymatic degradation

ESB ITA
https://doi.org/10.35119/maio.v2i2.67
Published 2018-06-18
Ahmed Kazaili
Department of Mechanical, Material and Aerospace Engineering, School of Engineering, University of Liverpool
Riaz Akhtar
Department of Mechanical, Material and Aerospace Engineering, School of Engineering, University of Liverpool
PDF

How to Cite

1.
Kazaili A, Akhtar R. Ultrastructural and nanomechanical changes of the cornea following enzymatic degradation. MAIO [Internet]. 2018 Jun. 18 [cited 2024 Apr. 19];2(2):24-9. Available from: https://www.maio-journal.com/index.php/MAIO/article/view/67
Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Copyright notice

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication, with the work twelve (12) months after publication simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.

  2. After 12 months from the date of publication, authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Keywords

amylase; corneal biomechanics; keratoconus

Abstract

Understanding of the ultrastructure and nanomechanical behavior of the cornea is important for a number of ocular disorders. In this study, atomic force microscopy (AFM) was used to determine nanoscale changes in the porcine cornea following enzymatic degradation. Diff erent concentrations of amylase were used to degrade the cornea. A reduction in elastic modulus at the nanoscale, along with disrupted collagen morphology, was observed following enzymatic treatment. This study highlights the interplay between mechanical properties and collagen organization in the healthy cornea.

https://doi.org/10.35119/maio.v2i2.67
PDF

References

Meek KM, Knupp C. Corneal structure and transparency. Prog Retin Eye Res. 2015;49:1-6.

Müller LJ, Pels E, Vrensen GF. The specific architecture of the anterior stroma accounts for maintenance of corneal curvature. Br J Ophthalmol. 2001;85(4):437-443.

Ambekar R, Toussaint KC, Johnson AW. The effect of keratoconus on the structural, mechanical, and optical properties of the cornea. J Mech Behav Biomed Mater. 2011;4(3):223-236.

Fischer AH, Jacobson KA, Rose J, Zeller R. Cryosectioning tissues. Cold Spring Harb Protoc. 2008;3(8). doi:10.1101/pdb.prot4991.

Papi M, Paoletti PA, Geraghty BR, Akhtar R. Nanoscale characterization of the biomechanical properties of collagen fibrils in the sclera. App Phys Lett. 2014;104(10):103703.

Pittenger B, Erina N, Su C. Quantitative mechanical property mapping at the nanoscale with Peak-Force QNM. Application Note Veeco Instruments Inc. 2010;1-2.

Dokukin ME, Sokolov I. Quantitative mapping of the elastic modulus of soft materials with HarmoniX and PeakForce QNM AFM modes. Langmuir. 2012;28(46):16060-16071.

PDF
1