Ultrastructural and nanomechanical changes of the cornea following enzymatic degradation

Ahmed Kazaili, Riaz Akhtar


Understanding of the ultrastructure and nanomechanical behavior of the cornea is important for a number of ocular disorders. In this study, atomic force microscopy (AFM) was used to determine nanoscale changes in the porcine cornea following enzymatic degradation. Diff erent concentrations of amylase were used to degrade the cornea. A reduction in elastic modulus at the nanoscale, along with disrupted collagen morphology, was observed following enzymatic treatment. This study highlights the interplay between mechanical properties and collagen organization in the healthy cornea.


amylase; corneal biomechanics; keratoconus

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Meek KM, Knupp C. Corneal structure and transparency. Prog Retin Eye Res. 2015;49:1-6.

Müller LJ, Pels E, Vrensen GF. The specific architecture of the anterior stroma accounts for maintenance of corneal curvature. Br J Ophthalmol. 2001;85(4):437-443.

Ambekar R, Toussaint KC, Johnson AW. The effect of keratoconus on the structural, mechanical, and optical properties of the cornea. J Mech Behav Biomed Mater. 2011;4(3):223-236.

Fischer AH, Jacobson KA, Rose J, Zeller R. Cryosectioning tissues. Cold Spring Harb Protoc. 2008;3(8). doi:10.1101/pdb.prot4991.

Papi M, Paoletti PA, Geraghty BR, Akhtar R. Nanoscale characterization of the biomechanical properties of collagen fibrils in the sclera. App Phys Lett. 2014;104(10):103703.

Pittenger B, Erina N, Su C. Quantitative mechanical property mapping at the nanoscale with Peak-Force QNM. Application Note Veeco Instruments Inc. 2010;1-2.

Dokukin ME, Sokolov I. Quantitative mapping of the elastic modulus of soft materials with HarmoniX and PeakForce QNM AFM modes. Langmuir. 2012;28(46):16060-16071.


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